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实验室常用荧光物质激发波长和发射波长

发布时间:2023-07-22 00:00:00      发布人:上海路阳   浏览量:784

实验室常用荧光物质激发波长和发射波长

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荧光物质 激发波长 发射波长
EX nm EX(Sub) EM nm
Alexa Fluor 532 532 554
Cy3 550 570
DsRed 557 579
EtBr 300 518 605
FITC 490 525
Gel Green 250 500 530
GFP 488 507
mCherry 580 610
SYBR Gold 495 540
SYBR Green I 498 522
SYPRO Red 550 300 630
SYPRO Ruby 280 450 620
TagRFP 555 583
Gel Red 270 510 600

■ AlexaFluor532激发波长为532nm,发射波长为554nm

■ Cy3激发波长为550nm,发射波长为570nm

■ DsRed-Express2 激发波长为557nm,发射波长为579nm

■ Ethidium Bromide 激发波长为300/518nm,发射波长为605nm

■ FITC激发波长为490nm,发射波长为525nm

■ Gel Green激发波长为500nm,发射波长为530nm

■ Gel Red激发波长为510nm,发射波长为600nm

■ GFP / EGFP 激发波长为488nm,发射波长为507nm

■ mCherry激发波长为580nm,发射波长为610nm

■ TagRFP激发波长为555nm,发射波长为583nm

■ SYBR Gold激发波长为490nm,发射波长为540nm

■ SYBR Green激发波长为498nm,发射波长为522nm

■ SYPRO Orange激发波长为470nm,发射波长为570nm

■ SYPRO Red激发波长为550nm,发射波长为630nm

■ SYPRO Ruby激发波长为450nm,发射波长为620nm

荧光物质名称 激发波长 发射波长
1, 5 IAEDANS 336nm 482nm
4-Methylumbelliferone 360nm 449nm
5-Carboxyfluorescein (5-FAM)® 492nm 518nm
5-Carboxynaphthofluorescein® 598nm 668nm
5-Carboxytetramethylrhodamine(5-TAMRA)® 542nm 568nm
5-FAM (5-Carboxyfluorescein)® 492nm 518nm
5-ROX (carboxy-X-rhodamine) 574nm 602nm
5-TAMRA (5-Carboxytetramethylrhodamine) 542nm 568nm
6-Carboxyrhodamine 6G (6-CR-6G)® 518nm 543nm
6-CR 6G® 518nm 543nm
6-JOE 525nm 555nm
7-Amino-4-methylcoumarin® 351nm 430nm
7-Aminoactinomycin D (7-AAD)® 546nm 647nm
7-Hydroxy-4-methylcoumarin (pH 9) 360nm 449nm
AcGFP 475nm 505nm
Acridine® orange(+DNA) 500nm 526nm
Acridine® orange(+RNA) 460nm 650nm
Alexa Fluor® 350 346nm 442nm
Alexa Fluor® 405 401nm 422nm
Alexa Fluor® 488 498nm 520nm
Alexa Fluor® 514 517nm 539nm
Alexa Fluor® 532 532nm 553nm
Alexa Fluor® 546 556nm 573nm
Alexa Fluor® 555 553nm 568nm
Alexa Fluor® 568 #4433c0 #5544cc 578nm 603nm
Alexa Fluor® 594 590nm 617nm
Alexa Fluor® 633 632nm 647nm
Alexa Fluor® 647 649nm 666nm
Alexa Fluor® 680 680nm 702nm
Alexa Fluor® 700 696nm 719nm
Alexa Fluor® 750 752nm 779nm
Alexa Fluor® 790 782nm 805nm
Alexa Fluor® 488/546 FRET 495nm 573nm
Alexa Fluor® 488/555 FRET 495nm 568nm
Alexa Fluor® 488/Cy3® FRET 495nm 570nm
Allophycocyanin (APC) 650nm 660nm
AMCA/AMCA-X 345nm 445nm
AmCyan 458nm 489nm
Aniline Bluer® 370nm 509nm
ANSr® 372nm 455nm
AsRed2 576nm 592nm
Atto 390 390nm 479nm
Atto 425 436nm 485nm
Atto 465 453nm 507nm
Atto 488 501nm 525nm
Atto 550 554nm 576nm
Atto 633 630nm 657nm
Atto 647N 644nm 669nm
Atto 680 680nm 700nm
ATTO-TAG™ CBQCA 465nm 560nm
ATTO-TAr™ FQ 486nm 591nm
Auraminer O-Feulgen 460nm 550nm
Azamir Green 492nm 505nm
BCECF 503nm 528nm
BCECF/pH 5.2 482nm 520nm
BCECF/pH 9.0 503nm 528nm
BFP (Blue Fluorescent Protein)r 382nm 448nm
BFP/DsRed2 FRET 382nm 583nm
BFP/eGFP FRET 382nm 508nm
BFP/YFP FRET 382nm 527nm
Biosearch Blue 352nm 447nm
BOBO™-1 BO-PRO™-1 462nm 481nm
BOBO™-3 BO-PRO™-3 570nm 604nm
BODIPY® FL/pH7.2 505nm 512nm
BODIPY® 530/550 533nm 550nm
BODIPY® 558/568 558nm 568nm
BODIPY® 564/570 564nm 570nm
BODIPY® 581/591 582nm 590nm
BODIPY® 630/650-X 630nm 650nm
BODIPY® 650/665-X 650nm 665nm
BODIPY® FL 505nm 513nm
BODIPY® TMR 542nm 574nm
BODIPY® TR-X 589nm 617nm
BTC 401nm 529nm
CAL Fluor® Gold 540 522nm 544nm
CAL Fluor® Orange 560 538nm 559nm
CAL Fluor® Red 590 569nm 591nm
CAL Fluor® Red 610 590nm 610nm
CAL Fluor® Red 635 618nm 637nm
Calcein 494nm 517nm
Calcein Blue 375nm 420nm
Calcein® 494nm 517nm
Calcium Crimson™ 590nm 615nm
Calcium Green-1™ 506nm 531nm
Calcium Orange™ 549nm 576nm
Calcofluor® White 350nm 440nm
Carboxy-X-rhodamine (5-ROX) 574nm 602nm
Cascade Blue® 400nm 420nm
Cascade Yellow™ 402nm 545nm
CCF2 (GeneBLAze™r) 402nm 520nm
Cerulean 433nm 475nm
CFP(Cyan Fluorescent Protein)? 434nm 477nm
CFP/DsRed2 FRET 434nm 583nm
CFP/YFP FRET 434nm 527nm
Chromomycin A3 450nm 470nm
Citrine 514nm 529nm
Cl-NERF (low pH) 504nm 540nm
Coumarin 384nm 470nm
C-phycocyanin 620nm 648nm
CPM 385nm 471nm
CryptoLight CF-2 584nm 657nm
CryptoLight CF-5 566nm 597nm
CryptoLight CF-6 566nm 615nm
CTC Formazan 450nm 630nm
Cy2™ 489nm 506nm
Cy3.5™ 581nm 598nm
Cy3™ /Cy5.5™ FRET 550nm 694nm
Cy3™ /Cy5™ FRET 550nm 670nm
Cy3™ 552nm 570nm
Cy5.5™ 675nm 694nm
Cy5™ 649nm 670nm
Cy7™ 743nm 767nm
Cycle 3 GFP 395nm 507nm
Dansyl cadaverine 335nm 518nm
Dansylchloride 380nm 475nm
DAPI 358nm 461nm
Dapoxyl® 373nm 574nm
Dendra2 Green 490nm 507nm
Dendra2 Red 553nm 573nm
Di-4 ANEPPS 488nm 605nm
Di-8 ANEPPS 468nm 635nm
Di-8-ANEPPS non-ratiometric 530nm 620nm
DiA 456nm 591nm
DiA (4-Di-16-ASP) 491nm 613nm
DiD (DilC18(5)) 644nm 665nm
DIDS? 341nm 414nm
DiI (DiIC18(3)) 549nm 565nm
DiO (DiOC18(3)) 484nm 501nm
DiR (DiIC18(7)) 750nm 779nm
DM-NERF (4.5_6.5 pH) 510nm 536nm
Draq5 647nm 683nm
Dronpa Green 503nm 518nm
DsRed2 (Red Fluorescent Protein) 558nm 583nm
DsRed-Express 557nm 579nm
DsRed-Monomer 556nm 586nm
DyLight 350 352nm 435nm
DyLight 405 399nm 433nm
DyLight 488 492nm 517nm
DyLight 549 554nm 569nm
DyLight 594 592nm 616nm
DyLight 633 623nm 645nm
DyLight 649 652nm 667nm
DyLight 680 677nm 705nm
DyLight 750 751nm 772nm
DyLight 800 770nm 795nm
EBFP 380nm 440nm
EBFP2 385nm 448nm
ECFP 433nm 475nm
EGFP 488nm 509nm
EGFP/DsRed 2 FRET 470nm 585nm
EGFP/Rhod-2 FRET 488nm 571nm
ELF®-97 alcohol 345nm 530nm
Emerald 487nm 509nm
EmGFP 487nm 509nm
EosFP Green 506nm 516nm
EosFP Red 571nm 581nm
Eosin 524nm 544nm
Erythrosin 529nm 554nm
Ethidium bromide 300nm 605nm
Ethidium homodimer-1 (EthD-1) 528nm 617nm
Europium (III) Chloride 337nm 613nm
EYFP 513nm 527nm
FAM 492nm 518nm
Fast Blue 365nm 420nm
FITC 490nm 525nm
FITC/Cy3® FRET 494nm 570nm
FITC/Rhod 2 FRET 494nm 571nm
FITC/TRITC FRET 494nm 580nm
FlAsH-CCPFCC 511nm 530nm
Fluo-3 506nm 526nm
Fluo-4 494nm 516nm
Fluorescein (FITC) 494nm 518nm
Fluoro-Gold (high pH) 368nm 565nm
Fluoro-Gold (low pH) 323nm 408nm
Fluoro-Jade 475nm 525nm
FluorX® 494nm 519nm
FM™ 1-43 479nm 598nm
FM™ 4-64 515nm 740nm
Fura Red™ Fluo-3 472nm 672nm
Fura Red™ 436nm 637nm
Fura-2 335nm 505nm
Fura-2/BCECF 335nm 528nm
Fura-2/Ca2+ - free 340nm 510nm
Fura-2/Ca2+ - saturated 380nm 510nm
FusionRed 576nm 603nm
Gel Green 250 530
Gel Red 270 600
GeneBLAzer™ (CCF2) 402nm 520nm
GFP 488nm 507nm
GFP (sapphire) 395nm 508nm
GFP/Rhod 2 FRET 488nm 571nm
GFP2 395nm 510nm
HcRed 588nm 618nm
HEX,SE 533nm 559nm
HiLyte Fluor™ 488 497nm 525nm
HiLyte Fluor™ 555 550nm 566nm
HiLyte Fluor™ 647 649nm 672nm
HiLyte Fluor™ 680 688nm 700nm
HiLyte Fluor™ 750 750nm 782nm
Hoechst 33258 352nm 461nm
Hoechst 33342 & 33258 352nm 461nm
HyPer 420nm 516nm
Indo-1 330nm 401nm
Indo-1/Ca2+ -free 346nm 475nm
Indo-1/Ca2+ -saturated 330nm 401nm
JC-1 498nm 595nm
JOE 520nm 548nm
JOJO™-1, JO-PRO™-1 529nm 545nm
JRed 584nm 610nm
Kaede Green 508nm 518nm
Kaede Red 572nm 580nm
KeimaRed 440nm 616nm
KikumeGR Green 507nm 517nm
KikumeGR Red 583nm 593nm
KillerRed 585nm 610nm
Kusabira Orange (mKO) 548nm 549nm
Li-Cor IRDye® 680LT 674nm 693nm
Li-Cor IRDye® 800CW 778nm 794nm
Lissamine rhodamine B 570nm 590nm
LOLO™-1, LO-PRO™-1 565nm 579nm
Lucifer Yellow 428nm 536nm
LysoSensor™ Blue (pH 5) 374nm 424nm
LysoSensor™ Green (pH 5) 442nm 505nm
LysoSensor™ Yellow/Blue (pH 4.2) 384nm 540nm
LysoTracker™ Blue/MeOH 373nm 425nm
LysoTracker™ Yellow HCK-123 486nm 563nm
LysoTracker™ Green 504nm 511nm
LysoTracker™ Red 577nm 592nm
LysoTracker™ Yellow 465nm 535nm
mAG (Azami?Green) 492nm 505nm
Mag-Fura-2 330nm 491nm
Mag-Indo-1 330nm 417nm
Magnesium Green™ 506nm 531nm
Marina Blue® 365nm 460nm
mBanana 540nm 553nm
mCherry 587nm 610nm
mCitrine 516nm 529nm
mHoneydew 487nm 537nm
Midoriishi-Cyan (MiCy) 472nm 495nm
MidoriishiCyan/KusabiraOrange FRET 472nm 559nm
Mithramycin 395nm 535nm
MitoTracker® Deep Red 633/MeOH 644nm 665nm
MitoTracker® Green 490nm 516nm
MitoTracker® Orange 551nm 576nm
MitoTracker® Red 578nm 599nm
mKate2 589nm 634nm
mKeima Red 440nm 620nm
mKO (Kusabira Orange) 548nm 549nm
mOrange 548nm 562nm
mPlum 590nm 649nm
mRasberry 598nm 625nm
mRFP1 584nm 607nm
mStrawberry 574nm 596nm
mTangerine 568nm 585nm
mTFP1 462nm 492nm
mTurquoise 434nm 474nm
mWasabi 493nm 509nm
NBD 465nm 535nm
Nile Blue 631nm 660nm
Nile Red 549nm 628nm
NirFP 604nm 669nm
Oregon Green™ 488 496nm 524nm
Oregon Green™ 500 503nm 522nm
Oregon Green™ 514 511nm 530nm
Oregon Green™ 514 506nm 526nm
Pacific Blue? 410nm 455nm
PBF1 334nm 504nm
PhiYFP 525nm 537nm
PhiYFP-m 525nm 537nm
PKH26 551nm 567nm
POPO™-1, PO-PRO™-1 434nm 456nm
POPO™-3, PO-PRO™-3 534nm 572nm
Propidium Iodide 536nm 617nm
PS-CFP 400nm 468nm
PS-GFP 490nm 511nm
Pulsar™ 650 460nm 650nm
PyMPO 415nm 570nm
Pyrene 345nm 378nm
Pyronin Y 555nm 580nm
Qdot™ 525 Conjugate UV 525nm
Qdot™ 565 Conjugate UV 565nm
Qdot™ 585 Conjugate UV 585nm
Qdot™ 605 Conjugate UV 605nm
Qdot™ 655 Conjugate UV 655nm
Qdot™ 705 Conjugate UV 705nm
Qdot™ 800 Co

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