312nm中波紫外灯LEB-280L用于白内障疾病研究

312nm中波紫外灯LEB-280L用于白内障疾病研究

哈尔滨医科大学发表文献《Melatonin inhibits ferroptosis and delays age-related cataract by regulating SIRT6/p-Nrf2/GPX4 and SIRT6/NCOA4/FTH1 pathways》，文献中的实验使用了美国路阳公司生产的312nm中波紫外灯LEB-280L，使用紫外灯LEB-280L输出不同剂量的紫外线辐照细胞，用LEB-280L模拟阳光中的紫外线，用来研究不同剂量的中波紫外线对细胞凋亡的影响。312nm中波紫外灯LEB-280L内置2根8W 312nm紫外线灯管，并安装了紫外滤光片，能够输出纯正的中波紫外线，没有UVA、UVC紫外线，并可以选购台式支架，满足实验的长时间辐照，具体紫外线灯LEB-280L产品介绍请浏览：《实验室用紫外线灯-LEB 系列手持式中波紫外线灯》。

请注意，操作紫外线灯请务必佩戴专业紫外线防护眼镜！

文献简介：

Introduction

Cataracts are the primary cause of preventable blindness globally. With the ageing of the world's population, the proportion of age-related cataract (ARC) patients will increase in both developed and developing countries. Surgical treatment of cataracts is the most effective method in the clinic. However, there is also a risk of intraoperative and postoperative complications, such as posterior capsule opacification, intraocular inflammation and corneal edema [2]. Among many kinds of environmental stresses, such as ultraviolet (UV) radiation, diabetes, drug intake, and excessive smoking and alcohol consumption, UV radiation is the most important risk factor for ARCs. It has been shown that cortical and posterior subcapsular cataracts can be caused dose-dependently by consistent ocular ultraviolet B (UVB) radiation exposure [4]. The formation of cataracts induced by UVB begins with cumulative oxidative damage to human lens epithelial cells (HLECs). During this process, the modes of cell death include apoptosis, necroptosis and pyroptosis.

Materials

Human lens epithelial cell line B-3 (B-3) was obtained from the American Type Culture Collection (ATCC Cat# CRL-11421, RRID: CVCL_6367), SRA01/04 and human embryonic kidney 293 T cell line (HEK-293 T) were purchased from Chinese Academy of Sciences (Shanghai, China). Foetal bovine serum (FBS) was purchased from Gibco (Thermo Fisher Scientific, Inc., Waltham, MA, USA), and Dulbecco's modified Eagle's medium was obtained from VivaCell Shanghai (Shanghai, China). UVB exposure was executed with a UVB radiometer (LUYOR-LEB-280L, USA) with a total output of 5 W/m2 (312 nm). A lux meter was used to test the irradiance of UV radiation. A Cell Counting Kit-8 (CCK-8) was purchased from Merck (Darmstadt, Germany). TRIzol and primers were obtained from Invitrogen (Eugene, Oregon, USA). Primary antibodies and fluorochrome-labeled secondary antibodies were purchased from Abcam (Cambridge, UK) and Proteintech (Chicago, IL, USA). All small molecular inhibitors and activators were obtained from MedChemExpress (Monmouth Junction, NJ, USA) and Cayman Chemical Company (Ann Arbor, MI, USA).

文献地址：https://doi.org/10.1016/j.biopha.2022.114048

文献下载：

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