中波紫外交联仪用于UVB损伤研究

近日，深圳市护家科技有限公司在《Frontiers in Pharmacology》发表了文献《Synergistic effects of retinol and retinyl palmitate in alleviating UVB-induced DNA damage and promoting the homologousrecombination repair in keratinocytes》，文献中的实验采用了上海路阳仪器有限公司生产的UCL-3500M紫外交联仪，UCL-3500M安装了6根15w UVB紫外线灯管，内置了紫外线传感器，能够自动测量辐照能量，精准控制实验过程辐照的紫外线能量，UCL-3500M紫外交联仪是科研院所、化妆品以及化妆品原材料企业进行光毒性实验的非常好用的实验设备。

深圳市护家科技有限公司是深圳的一家高科技公司，正式成立于2014年7月，护家科技旗下拥有科研级功效护肤品牌HBN，HBN坚持以“拥有看得见效果”的创新理念，专研打造卓越功效的护肤品。致力于让用户把每一分钱都花在有效成分上 。深圳护家自建具有CNAS认证的科研实验室，包括细胞、理化、 植物原料、配方机制、临床功效等科研实验室，坚持自主创新研发。核心研发团队来自国内外知名科研院所工科博士、硕士等尖端科研专家团队。

文献摘要：

Background: Ultraviolet B (UVB) rays are a type of ultraviolet radiation emitted by the sun, primarily responsible for skin photodamage. These rays mainly affect the epidermis, leading to direct damage to DNA and contributing to skin cancer development. Retinol and its derivatives are effective in combating skin aging and photodamage, but they often cause skin intolerance, limiting their use despite their potent effects. Therefore, investigating optimal compositions of retinoids is essential to enhance their efficacy against photodamage.

Method: In this study, we investigated the synergistic effects of retinol (ROL) and retinyl palmitate (RPalm) in alleviating UVB-induced DNA damage in human keratinocytes (HaCaT) and reconstructed human epidermis. The ROL+RPalm combination was applied after UVB exposure. We utilized bulk mRNA sequencing, comet assays, Western blotting, immunofluorescence, and flow cytometry to evaluate the level of DNA damage and repair.

Result: The application of the ROL+RPalm combination significantly reduced inflammation and apoptosis while promoting collagen synthesis compared to individual treatments with ROL or RPalm. Our findings indicated that the ROL+RPalm synergy primarily mediates DNA damage repair. Additionally, we elucidated that the molecular mechanism involves the activation of RARβ, which triggers the ATM-CHK2-p53 signaling pathway and increases the expression of homologous recombination (HR)-associated repair genes. Conclusion: This combination of ROL and RPalm presents a potential therapeutic strategy for UVB-induced photodamage and emphasizes the synergistic effects in alleviating UVB-induced DNA damage。

文献全文下载：

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文献地址：https://doi.org/10.3389/fphar.2025.1562244