便携式荧光蛋白激发光源用于观察dsred的表达

便携式荧光蛋白激发光源用于观察dsred的表达

近日聊城大学农学院在《Agronomy》期刊上发表《Agrobacterium rhizogenes-Mediated Genetic Transformation and Establishment of CRISPR/Cas9 Genome-Editing Technology in Limonium bicolor》，文献中实验使用了LUYOR-3415RG便携式双波长荧光蛋白激发光源，用于观察dsred在植物根系的表达。

上图：左侧为白光下的观察效果，

右侧F图：在LUYOR-3415RG便携式激发光源照射下左侧的有明显的dsred的红色荧光，右侧为没有荧光表达。

文献摘要：

Limonium bicolor is a perennial herbaceous plant belonging to the Plumbaginaceae family. It can be used as a dried flower or in cut flower arrangements and serves as a model recretohalophyte. Its genome sequencing has been recently completed. However, the research on L. bicolor is limited by the absence of a highly efficient genetic transformation system. In this study, we established a highly efficient Agrobacterium rhizogenes-mediated L. bicolor genetic transformation method. The transgenic hairy roots were induced from the hypocotyl of L. bicolor using A. rhizogenes strain K599 harboring pRdGa4Cas9 plasmid (which carries an expression cassette of 35S::DsRed2). The transgenic shoots were regenerated from hairy root segments (~0.1 cm diameter), and induction efficiency was achieved at . The transgenic shoots with 4–5 rosette leaves were directly planted into the soil to induce the transgenic roots. Therefore, transgenic plantlets were produced. The DsRed2 can be used as a reliable reporter gene in screening transgenic plantlets. Furthermore, we also established a CRISPR/Cas9 system in L. bicolor employing the A. rhizogenes-mediated genetic transformation approach. The highly efficient transformation method and CRIPSP/Cas9 system established will provide a valuable tool for functional genomics investigation and trait improvement in L. bicolor.

文献地址：https://doi.org/10.3390/agronomy13092244   

美国路阳生产的LUYOR-3415系列便携式双波长荧光蛋白激发光源在中国有数千台的销售，每年都有大量文献发布在 期刊上，LUYOR-3415便携式激发光源能够观察gfp、egfp、bfp、yfp、rfp等各种荧光蛋白在植物叶片、种子、愈伤以及各种模式动物上的表达。如果您对便携式激发光源还不是很了解，我们提供样机免费试用。